Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8793] to WDR1 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-WDR1 antibody [EPR8793] - BSA and Azide free
    See all WDR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8793] to WDR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human heart tissue lysate. Jurkat, Raji, HT-1080 and U87-MG cell lysates. IHC: Human skeletal muscle tissue and endometrial carcinoma tissue. ICC/IF: Raji cells.

  • General notes

    ab249772 is the carrier-free version of ab173574.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    All lanes : Anti-WDR1 antibody [EPR8793] (ab173574) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 66 kDa



    This data was developed using ab173574, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    This data was developed using ab173574, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with ab173574 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    This data was developed using ab173574, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with ab173574 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Immunocytochemistry - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    This data was developed using ab173574, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Raji cells labeling WDR1 with ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).
  • Immunocytochemistry - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Immunocytochemistry - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    Immunofluorescence analysis of Raji cells labeling WDR1 with ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with ab173574 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with ab173574 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    All lanes : Anti-WDR1 antibody [EPR8793] (ab173574)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
    Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa



    Buffer concentration: 5% NFDM /TBST.

    We are unsure as to the identity of the extra band below 75kDa.

  • Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Anti-PDK2 antibody [EPR1948Y] (ab68164) at 1/200 dilution + Human heart tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (HRP), with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 66 kDa


    Exposure time: 180 seconds


    Buffer concentration: 5% NFDM /TBST

    For human samples, please use lower antibody dilution or upload more of the protein.

  • Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)
    Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

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