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Cardiovascular Atherosclerosis Thrombosis Platelets

Human CD63 Antibody Pair - BSA and Azide free (ab253430)

Human CD63 Antibody Pair - BSA and Azide free (ab253430)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human CD63 Antibody Pair - BSA and Azide free
    See all CD63 kits
  • Assay type

    ELISA set
  • Range

    78.125 pg/ml - 5000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human CD63. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab275099), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human CD63 Capture Antibody (unconjugated) 1 x 100µg
    Human CD63 Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
  • Function

    This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • Tissue specificity

    Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization

    Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Target information above from: UniProt accession P08962 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Lysosomal associated membrane protein 3
    • CD 63
    • CD63
    • CD63 antigen
    • CD63 antigen (melanoma 1 antigen)
    • CD63 antigen melanoma 1 antigen
    • CD63 molecule
    • CD63_HUMAN
    • gp55
    • Granulophysin
    • LAMP 3
    • LAMP-3
    • LAMP3
    • LIMP
    • Lysosomal-associated membrane protein 3
    • Lysosome associated membrane glycoprotein 3
    • Lysosome-associated membrane glycoprotein 3
    • Mast cell antigen AD1
    • ME491
    • Melanoma 1 antigen
    • Melanoma associated antigen ME491
    • Melanoma associated antigen MLA1
    • Melanoma-associated antigen ME491
    • MGC72893
    • MLA 1
    • MLA1
    • NGA
    • Ocular melanoma associated antigen
    • Ocular melanoma-associated antigen
    • OMA81H
    • PTLGP40
    • Tetraspanin 30
    • Tetraspanin-30
    • Tspan 30
    • Tspan-30
    • TSPAN30
    see all
  • Database links

    • Entrez Gene: 967 Human
    • Omim: 155740 Human
    • SwissProt: P08962 Human
    • Unigene: 445570 Human

    Images

    • Sandwich ELISA - Human CD63 Antibody Pair - BSA and Azide free (ab253430)
      Sandwich ELISA - Human CD63 Antibody Pair - BSA and Azide free (ab253430)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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