Human CD63 ELISA Kit (ab275099)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 33 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD63 ELISA Kit
See all CD63 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 7 6.4% Inter-assay Sample n Mean SD CV% Serum 3 7.7% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
33 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 96% - 98% Serum 97 94% - 101% EDTA Plasma 94 90% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD63 ELISA kit (ab275099) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD63 protein in cell culture supernatant, plasma EDTA, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD63 with 33 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human CD63 antigen, also referred to as Granulophysin, Lysomal-associated membrane protein 3 (LAMP-3), Tetraspanin-30, and Ocular melanoma-associated antigen, is a membrane bound protein encoded by the gene CD63. CD63 belongs to a group of proteins known as the tetraspanin family, which are characterized by the presence of four hydrophobic domains and are found to mediate signal transduction events for the regulation of cell development, activation, growth, and motility. Deficiency of this protein is associated with Hermansky-Pudlak Syndrome, which is an autosomal disorder.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD63 Capture Antibody 1 x 600µl 10X Human CD63 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CD63 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Alternative names
- Lysosomal associated membrane protein 3
- CD 63
- CD63
see all -
Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD63 standard curve in Sample Diluent NS.The CD63 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Serum from eight individual healthy CD63 female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD63 concentration was determined to be 252 pg/mL with a range of 103 – 408 pg/mL.
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Sandwich ELISA - Human CD63 ELISA Kit (ab275099)To learn more about the advantages of recombinant antibodies see here.
