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Mouse CD31 Antibody Pair - BSA and Azide free (ab241693)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Mouse

Overview

  • Product name

    Mouse CD31 Antibody Pair - BSA and Azide free
    See all CD31 kits

  • Assay type

    ELISA set

  • Range

    31 pg/ml - 2000 pg/ml

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    The Antibody Pair can be used to quantify Mouse CD31. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab204527), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Mouse CD31 Capture Antibody (unconjugated) 1 x 100µg
    Mouse CD31 Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

  • Tissue specificity

    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).

  • Sequence similarities

    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

  • Domain

    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.

  • Post-translational
    modifications

    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.

  • Cellular localization

    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt

  • Alternative names

    • Adhesion molecule
    • CD31
    • CD31 antigen
    • CD31 EndoCAM
    • EndoCAM
    • FLJ34100
    • FLJ58394
    • GPIIA
    • GPIIA'
    • PECA1
    • PECA1_HUMAN
    • PECAM
    • Pecam 1
    • PECAM 1 CD31 EndoCAM
    • PECAM-1
    • Pecam1
    • Platelet and endothelial cell adhesion molecule 1
    • Platelet endothelial cell adhesion molecule
    • Platelet/endothelial cell adhesion molecule 1
    see all

  • Database links

    Images

    • Sandwich ELISA - Mouse CD31 Antibody Pair - BSA and Azide free (ab241693)
      Sandwich ELISA - Mouse CD31 Antibody Pair - BSA and Azide free (ab241693)
      To learn more about the advantages of recombinant antibodies see here.

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