Anti-Nectin 3 antibody [EPR24274-4] (ab278526)
![Anti-Nectin 3 antibody [EPR24274-4] (ab278526)](https://www.abcam.com/ps/products/278/ab278526/Images/ab278526-450344-anti-nectin-3-antibody-epr242744-western-blot-colorectal.jpg "Anti-Nectin 3 antibody [EPR24274-4] (ab278526)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24274-4] to Nectin 3
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Nectin 3 antibody [EPR24274-4]
See all Nectin 3 primary antibodies -
Description
Rabbit monoclonal [EPR24274-4] to Nectin 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HCT 116 and MDA-MB-231 whole cell lysate. IP: MDA-MB-231 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24274-4 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nectin 3 antibody [EPR24274A-4] (ab278526)All lanes : Anti-Nectin 3 antibody [EPR24274-4] (ab278526) at 1/1000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 61 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID:11024295, 26177744).
Negative control: U-937 (PMID: 11024295).
Exposure time: 158 seconds
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![Immunoprecipitation - Anti-Nectin 3 antibody [EPR24274A-4] (ab278526)](https://www.abcam.com/ps/products/278/ab278526/Images/ab278526-1-anti-nectin-3-antibody-epr242744-immunoprecipitation-mdamb231.jpg)
Immunoprecipitation - Anti-Nectin 3 antibody [EPR24274A-4] (ab278526)Nectin 3 was immunoprecipitated from 0.35 mg MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab278526 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278526 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MDA-MB-231 whole cell lysate 10 µg
Lane 2: ab278526 IP in MDA-MB-231 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278526 in MDA-MB-231 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 119 seconds