Anti-Tollip antibody (ab187198)
Key features and details
- Rabbit polyclonal to Tollip
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Tollip antibody
See all Tollip primary antibodies -
Description
Rabbit polyclonal to Tollip -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow -
Immunogen
Recombinant full length protein corresponding to Human Tollip aa 1 to the C-terminus.
Database link: Q9H0E2 -
Positive control
- WB: SW480, OVCAR3, MCF-7 and 293T cell lysates, mouse spleen, intestine and liver tissue lysates; IHC: Rat brain; ICC/IF: L929 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded rat brain tissue stained for Tollip using ab187198 at 1/100 dilution in immunohistochemical analysis.
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Immunofluorescence staining of L929 cells stained for Tollip with ab187198 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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All lanes : Anti-Tollip antibody (ab187198) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : OVCAR3 cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : Mouse spleen tissue lysate
Lane 6 : Mouse intestine tissue lysate
Lane 7 : Mouse liver tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 30 kDaBlocking buffer: 3% nonfat dry milk in TBST.