Anti-Tollip antibody (ab37155)
Key features and details
- Rabbit polyclonal to Tollip
- Suitable for: IHC-P, WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Tollip antibody
See all Tollip primary antibodies -
Description
Rabbit polyclonal to Tollip -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to 16 amino acids near to the C terminus of the human Tollip protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tollip antibody (ab37155)Lane 1 : Anti-Tollip antibody (ab37155) at 1 µg/ml
Lane 2 : Anti-Tollip antibody (ab37155) at 2 µg/ml
All lanes : Rat brain cell lysate
Predicted band size: 30 kDa
Observed band size: 30 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling Tollip with Anti-Tollip antibody (ab37155) at 20μg/ml. -
Immunocytochemistry - Anti-Tollip antibody (ab37155)ab37155 at 2 µg/ml staining Tollip in THP-1 cells by ICC. Secondary antibody was biotin conjugated donkey anti-rabbit. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)ab37155 staining Tollip in human placenta.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
