Anti-LAMP1 antibody (ab62562)
Key features and details
- Rabbit polyclonal to LAMP1
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LAMP1 antibody
See all LAMP1 primary antibodies -
Description
Rabbit polyclonal to LAMP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A 15 amino acid peptide from near the centre of human LAMP1.
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Positive control
- ICC: Human colon cells. WB: Mouse EL4 cell lysate. IHC-P: Rat colon tissue and human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody (ab62562)
Immunofluorescence of LAMP-1 in Human Colon cells using ab62562 at 20 ug/mL.
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Western blot - Anti-LAMP1 antibody (ab62562)Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml
All lanes : EL4 cell lysate
Predicted band size: 45 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody (ab62562)Immunohistochemistry of LAMP-1 in rat colon tissue with ab62562 at 2.5 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody (ab62562)Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requi
