Anti-LAMP1 antibody - Lysosome Marker (ab30687)
Key features and details
- Rabbit polyclonal to LAMP1 - Lysosome Marker
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
-
Product name
Anti-LAMP1 antibody - Lysosome Marker
See all LAMP1 primary antibodies -
Description
Rabbit polyclonal to LAMP1 - Lysosome Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Drosophila melanogaster
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) LAMP1.
Read Abcam's proprietary immunogen policy (Peptide available as ab32341.)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Drosophila melanogasterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120-140 kDa).Detects a band of approximately 120 kDa (predicted molecular weight: 35 kDa for the core protein but an expected size of 120-140 kDa due to glycosylation).
Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120-140 kDa).Detects a band of approximately 120 kDa (predicted molecular weight: 35 kDa for the core protein but an expected size of 120-140 kDa due to glycosylation).
Target
-
Function
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
-
Alternative names
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
Images
-
Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab30687)Anti-LAMP1 antibody - Lysosome Marker (ab30687) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 120-140 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab30687)Anti-LAMP1 antibody - Lysosome Marker (ab30687) at 1 µg/ml +Schneider L2 whole cell lysate (ab14893) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120-140 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab30687 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
References (16)
ab30687 has been referenced in 16 publications.
- Bergkvist L et al. Mapping pathogenic processes contributing to neurodegeneration in Drosophila models of Alzheimer's disease. FEBS Open Bio 10:338-350 (2020). PubMed: 31823504
- Aparicio R et al. Upregulation of the Autophagy Adaptor p62/SQSTM1 Prolongs Health and Lifespan in Middle-Aged Drosophila. Cell Rep 28:1029-1040.e5 (2019). PubMed: 31340141
- Wang R et al. ATP13A2 facilitates HDAC6 recruitment to lysosome to promote autophagosome-lysosome fusion. J Cell Biol 218:267-284 (2019). PubMed: 30538141
- Mondragon AA et al. Lysosomal Machinery Drives Extracellular Acidification to Direct Non-apoptotic Cell Death. Cell Rep 27:11-19.e3 (2019). PubMed: 30943394
- Lee KA et al. Inflammation-Modulated Metabolic Reprogramming Is Required for DUOX-Dependent Gut Immunity in Drosophila. Cell Host Microbe 23:338-352.e5 (2018). PubMed: 29503179
Images
-
Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab30687)Anti-LAMP1 antibody - Lysosome Marker (ab30687) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 120-140 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab30687)Anti-LAMP1 antibody - Lysosome Marker (ab30687) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120-140 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab30687 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
