Anti-CD1E antibody [EPR15746(B)] (ab187157)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15746(B)] to CD1E
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-CD1E antibody [EPR15746(B)]
See all CD1E primary antibodies -
Description
Rabbit monoclonal [EPR15746(B)] to CD1E -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatHumanWB MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HUVEC, HepG2 and Jurkat cell lysate. Raw264.7, NIH3T3 and PC-12 cell lysate. Human hepatocellular carcinoma tissue and rat kidney tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15746(B) -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-CD1E antibody [EPR15746(B)] (ab187157) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 44 kDaProduced under U.S. Patent No. 5,675,063.
-
All lanes : Anti-CD1E antibody [EPR15746(B)] (ab187157) at 1/1000 dilution
Lane 1 : Raw264.7 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 44 kDaProduced under U.S. Patent No. 5,675,063.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1E antibody [EPR15746(B)] (ab187157)
Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue sections labeling CD1E using ab187157 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1E antibody [EPR15746(B)] (ab187157)
Immunohistochemical analysis of paraffin embedded rat kidney tissue sections labeling CD1E using ab187157 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-