Anti-DNA Polymerase epsilon p59 antibody (ab241943)
Key features and details
- Rabbit polyclonal to DNA Polymerase epsilon p59
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA Polymerase epsilon p59 antibody
See all DNA Polymerase epsilon p59 primary antibodies -
Description
Rabbit polyclonal to DNA Polymerase epsilon p59 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human DNA Polymerase epsilon p59 aa 125-175. The exact sequence is proprietary.
Database link: P56282 -
Positive control
- IP: DNA Polymerase epsilon p59 IP in HEK-293T whole cell lysate.
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General notes
ab241943 has not performed satisfactorily when used for WB of DNA Polymerase epsilon p59 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of DNA Polymerase epsilon p59.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to DNA Polymerase epsilon p59 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-DNA Polymerase epsilon p59 antibody (ab241943)
DNA Polymerase epsilon p59 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241943 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.Lane 1: ab241943 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 10 seconds.
