Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9584] to CMT2
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-CMT2 antibody [EPR9584]
See all CMT2 primary antibodies
Description

Rabbit monoclonal [EPR9584] to CMT2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CMT2 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q15013
Positive control
- Recombinant Human CMT2 (ab123183) can be used as a positive control in WB. HepG2, A431, and HeLa cell lysates, Human brain and Human cervical carcinoma tissues This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
General notes
This product was previously labelled as MAD2L1 binding protein

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9584
Isotype

IgG
Research areas

Western blot - Anti-CMT2 antibody [EPR9584] (ab150363)

All lanes : Anti-CMT2 antibody [EPR9584] (ab150363) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 31 kDa
Observed band size: 34 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-CMT2 antibody [EPR9584] (ab150363)

ab150363 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab150363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] (ab150363)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CMT2 binding protein with ab150363 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] (ab150363)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling CMT2 binding protein with ab150363 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-CMT2 antibody [EPR9584] (ab150363)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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