Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/1000 dilution + Full length, with N-terminal GST and C-terminal His tag CDT2 (DTL) Human Recombinant Protein

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 79 kDa

Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling CDT2/RAMP using ab184548 at a 1/240 dilution (red) with negative control, Rabbit monoclonal IgG (green). Secondary antibody, Goat anti rabbit IgG (FITC) at a 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184548).

Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling CDT2/RAMP using ab184548 at a 1/100 dilution and Goat anti-rabbit IgG (Alexa Fluor^® 555) secondary at a 1/200 dilution (red). Counterstained with DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184548).

Immunohistochemical analysis of paraffin-embedded sections of human thymus tissue labeling CDT2/RAMP using ab184548 at a 1/100 dilution, counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184548).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded sections of human testis tissue labeling CDT2/RAMP using ab184548 at a 1/100 dilution, counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184548).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.