Anti-CDT2/RAMP antibody (ab174385)
Key features and details
- Rabbit polyclonal to CDT2/RAMP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDT2/RAMP antibody
See all CDT2/RAMP primary antibodies -
Description
Rabbit polyclonal to CDT2/RAMP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Recombinant fragment (His-tag) within Human CDT2/RAMP (C terminal). The exact sequence is proprietary.
Database link: 51514 -
General notes
This product was previously labelled as CDT2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling CDT2/RAMP with ab174385 at 1/100 dilution (Red). The cells were stained for DNA with Hoechst 33258 (Blue). CDT2 protein is localized in nuclei at all cell stages.
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Inhibition of CDT2/RAMP protein synthesis by CDT2/RAMP siRNA introduced into HeLa cells. siCont is control siRNA unrelated to CDT2/RAMP. siCDT2 is CDT2/RAMP specific siRNA.
CDT2/RAMP is phosphorylated after UV irradiation as shown by the band shift-up in irradiated sample (UV +).
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Anti-CDT2/RAMP antibody (ab174385) at 1/2000 dilution + HeLa whole cell extract at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 79 kDa