Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14978] to CDT2/RAMP
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CDT2/RAMP antibody [EPR14978]
    See all CDT2/RAMP primary antibodies

  • Description

    Rabbit monoclonal [EPR14978] to CDT2/RAMP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, Jurkat and HeLa whole cell lysate (ab150035). Full length, with N-terminal GST and C-terminal His tag CDT2 (DTL) Human Recombinant Protein. IHC-P: Human thymus and testis tissue. ICC/IF: HeLa cells.

  • General notes

     

     

     This product was previously labelled as CDT2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/10000 dilution + Full length, with N-terminal GST and C-terminal His tag CDT2 (DTL) Human Recombinant Protein

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 79 kDa
    Observed band size: 116 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa

  • Flow Cytometry - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Flow Cytometry - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

    Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling CDT2/RAMP using ab184548 at a 1/240 dilution (red) with negative control, Rabbit monoclonal IgG (green). Secondary antibody, Goat anti rabbit IgG (FITC) at a 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Immunocytochemistry/ Immunofluorescence - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

    Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling CDT2/RAMP using ab184548 at a 1/100 dilution and Goat anti-rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution (red). Counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

    Immunohistochemical analysis of paraffin-embedded sections of human testis tissue labeling CDT2/RAMP using ab184548 at a 1/100 dilution, counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

    Immunohistochemical analysis of paraffin-embedded sections of human thymus tissue labeling CDT2/RAMP using ab184548 at a 1/100 dilution, counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    All lanes : Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/50000 dilution

    Lane 1 : HEK-293 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa

  • Anti-CDT2/RAMP antibody [EPR14978] (ab184548)
    Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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