Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14978] to CDT2/RAMP
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-CDT2/RAMP antibody [EPR14978]
See all CDT2/RAMP primary antibodies
Description

Rabbit monoclonal [EPR14978] to CDT2/RAMP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293, Jurkat and HeLa whole cell lysate (ab150035). Full length, with N-terminal GST and C-terminal His tag CDT2 (DTL) Human Recombinant Protein. IHC-P: Human thymus and testis tissue. ICC/IF: HeLa cells.
General notes
This product was previously labelled as CDT2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14978
Isotype

IgG
Research areas

Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/10000 dilution + Full length, with N-terminal GST and C-terminal His tag CDT2 (DTL) Human Recombinant Protein

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 79 kDa
Observed band size: 116 kDa
why is the actual band size different from the predicted?
Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/10000 dilution + HeLa cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 79 kDa
Flow Cytometry - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling CDT2/RAMP using ab184548 at a 1/240 dilution (red) with negative control, Rabbit monoclonal IgG (green). Secondary antibody, Goat anti rabbit IgG (FITC) at a 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling CDT2/RAMP using ab184548 at a 1/100 dilution and Goat anti-rabbit IgG (Alexa Fluor^® 555) secondary at a 1/200 dilution (red). Counterstained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Immunohistochemical analysis of paraffin-embedded sections of human testis tissue labeling CDT2/RAMP using ab184548 at a 1/100 dilution, counterstained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Immunohistochemical analysis of paraffin-embedded sections of human thymus tissue labeling CDT2/RAMP using ab184548 at a 1/100 dilution, counterstained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

All lanes : Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/50000 dilution

Lane 1 : HEK-293 cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 79 kDa
Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com