Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)
![Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)](https://www.abcam.com/ps/products/253/ab253190/Images/ab253190-395299-anti-calcium-channel-l-type-dhpr-alpha-2-subunitcacna2d1-antibody-epr232678-western-blot-mouse-brain-tissue-lysate-20-ug.jpg "Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23267-8] to Calcium channel L type DHPR alpha 2 subunit/CACNA2D1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8]
See all Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 primary antibodies -
Description
Rabbit monoclonal [EPR23267-8] to Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat skeletal muscle, brain and heart tissue lysates; C2C12, C6 and PC-12 whole cell lysates. IHC-P: Rat skeletal muscle, pancreas and cerebrum tissue; Mouse skeletal muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23267-8 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)All lanes : Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Observed molecular weight disparity due to alternative splicing what has been described in the literature (PMID: 15000524).
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)](https://www.abcam.com/ps/products/253/ab253190/Images/ab253190-1-anti-calcium-channel-l-type-dhpr-alpha-2-subunitcacna2d1-antibody-epr232678-immunohistochemistry-mouse-skeletal-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse skeletal muscle (PMID: 18063658). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)](https://www.abcam.com/ps/products/253/ab253190/Images/ab253190-395298-anti-calcium-channel-l-type-dhpr-alpha-2-subunitcacna2d1-antibody-epr232678-western-blot-mouse-skeletal-muscle-tissue-lysate-20-ug.jpg)
Western blot - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)All lanes : Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190) at 1/1000 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lanes 1-4: 3 minutes; Lane 5: 15 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)](https://www.abcam.com/ps/products/253/ab253190/Images/ab253190-3-anti-calcium-channel-l-type-dhpr-alpha-2-subunitcacna2d1-antibody-epr232678-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat cerebrum (PMID: 8955374). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)](https://www.abcam.com/ps/products/253/ab253190/Images/ab253190-4-anti-calcium-channel-l-type-dhpr-alpha-2-subunitcacna2d1-antibody-epr232678-immunohistochemistry-rat-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat pancreatic islet (PMID: 28115397). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)](https://www.abcam.com/ps/products/253/ab253190/Images/ab253190-2-anti-calcium-channel-l-type-dhpr-alpha-2-subunitcacna2d1-antibody-epr232678-immunohistochemistry-rat-skeletal-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat skeletal muscle (PMID: 18063658). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)](https://www.abcam.com/ps/products/253/ab253190/Images/ab253190-7-benefits-of-recombinant-antibodies.png)
Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] (ab253190)
