Anti-Wnt4 antibody (ab262696)
Key features and details
- Rabbit polyclonal to Wnt4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt4 antibody
See all Wnt4 primary antibodies -
Description
Rabbit polyclonal to Wnt4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Wnt4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P56705 -
Positive control
- WB: HEK-293T and HepG2 whole cell extracts; IHC-P: Mouse kidney tissue and U87 xenograft.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Wnt4 antibody (ab262696)All lanes : Anti-Wnt4 antibody (ab262696) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 39 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt4 antibody (ab262696)Paraffin-embedded mouse kidney tissue stained Wnt4 using ab262696 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15mins.
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Western blot - Anti-Wnt4 antibody (ab262696)All lanes : Anti-Wnt4 antibody (ab262696) at 1/2500 dilution
Lane 1 : Wnt 2 transfected HEK 293T whole cell extract
Lane 2 : Wnt 2b transfected HEK 293T whole cell extract
Lane 3 : Wnt 3 transfected HEK 293T whole cell extract
Lane 4 : Wnt 4 (with V5-tag) transfected HEK 293T whole cell extract
Lane 5 : Wnt 5a transfected HEK 293T whole cell extract
Lane 6 : Wnt 7a transfected HEK 293T whole cell extract
Lane 7 : Wnt 7b transfected HEK 293T whole cell extract
Lane 8 : Wnt 8b transfected HEK 293T whole cell extract
Lane 9 : Wnt 9a transfected HEK 293T whole cell extract
Lane 10 : Wnt 9b transfected HEK 293T whole cell extract
Lane 11 : Wnt 10b transfected HEK 293T whole cell extract
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 39 kDaTransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (100 µg) were separated by 10% SDS-PAGE.
Bottom panel is internal control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt4 antibody (ab262696)Paraffin-embedded u87 xenograft tissue stained Wnt4 using ab262696 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15mins.
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Western blot - Anti-Wnt4 antibody (ab262696)All lanes : Anti-Wnt4 antibody (ab262696) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Wnt4 transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 39 kDa10%SDS PAGE
