Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free
See all CRCP primary antibodies
Description

Rabbit monoclonal [EPR9670(B)] to CRCP - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab248873 is the carrier-free version of ab139264. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248873 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9670(B)
Isotype

IgG
Research areas

Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/5000 dilution (unpurified)

Lane 1 : Jurkat cell lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 20 kDa
why is the actual band size different from the predicted?

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/10000 dilution (purified)

Lane 1 : Jurkat cell lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/1000 dilution (unpurified)

Lane 1 : Human ovary cancer lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lane 4 : Human prostate cancer lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 14 kDa

This data was developed using ab139264, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with purified ab139264 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CRCP with unpurified ab139264 at 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with unpurified ab139264 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunocytochemistry - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with purified ab139264 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunocytochemistry - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CRCP with unpurified ab139264 at 1/100.
Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.ab139264 (unpurified) at 1/50 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.ab139264 (purified) at 1/100 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)