Anti-Connexin 43 / GJA1 antibody (ab235282)
Key features and details
- Rabbit polyclonal to Connexin 43 / GJA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-Connexin 43 / GJA1 antibody
See all Connexin 43 / GJA1 primary antibodies -
Description
Rabbit polyclonal to Connexin 43 / GJA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: NIH/3T3 cells. Rat pup brain cells. IHC-P: Mouse heart tissue. WB: Mouse heart and rat brain tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized rat pup brain cells labeling Connexin 43 / GJA1 with ab235282 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green). The nuclear counterstain is DAPI (blue).
-
Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Connexin 43 - GJA1 with ab235282 at 1/200 dilution (30 minutes at RT), followed by a ready to use anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.Panel A: Mouse heart H&E.
Panel B: ab235282 at 20X magnification.
Panel C: ab235282 at 40X magnification. -
All lanes : Anti-Connexin 43 / GJA1 antibody (ab235282) at 1/1000 dilution
Lane 1 : Mouse heart tissue
Lane 2 : Rat brain tissue
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-rabbit-HRP at 1/70000 dilution
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Exposure time: 0.5 secs.
-
Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Connexin 43 / GJA1 with ab235282 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green). The nuclear counterstain is DAPI (blue).