Human Galectin 10 ELISA Kit (ab277447)
Key features and details
- Sensitivity: 0.45 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Galectin 10 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.45 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 77.23 72% - 83% Plasma 81.87 74% - 89% Cell culture media 84.75 74% - 93% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Galectin 10 ELISA Kit (ab277447) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 10 in serum (human Galectin 10 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human Galectin 10 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Galectin 10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Galectin 10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Galectin 10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human Galection 10 Antibody 2 vials Anti-Human Galectin 10 coated Microplate 1 unit Human Galectin 10 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May have both lysophospholipase and carbohydrate-binding activities. -
Tissue specificity
Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Cytoplasmic granule. Localized in granules from where it may be secreted or transported to other locations in the cell. - Information by UniProt
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Alternative names
- BSF3
- Charcot Leyden crystal protein
- Charcot-Leyden crystal protein
see all -
Database links
- Entrez Gene: 1178 Human
- Omim: 153310 Human
- SwissProt: Q05315 Human
- Unigene: 889 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.