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Human Myeloperoxidase ELISA Kit (ab272101)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 33.37 pg/ml
  • Range: 93.75 pg/ml - 6000 pg/ml
  • Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Myeloperoxidase ELISA Kit
    See all Myeloperoxidase kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 6 6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 6.1%

  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Cell culture extracts, Cell culture media, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    33.37 pg/ml

  • Range

    93.75 pg/ml - 6000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99 94% - 104%
    Saliva 103 94% - 118%
    Urine 89 85% - 95%
    Serum 91 88% - 94%
    Cell culture extracts 104 99% - 108%
    Cell culture media 94 86% - 98%
    EDTA Plasma 89 86% - 92%
    Cit plasma 88 86% - 91%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Myeloperoxidase Kit (ab272101) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myeloperoxidase protein in human serum, plasma, cell culture supernatant, urine, milk, saliva, cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Myeloperoxidase with 33.37 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Human Myeloperoxidase (MPO) is a hemoprotein expressed in the neutrophils (polymorphonuclear leukocytes) and is secreted during activation. Myeloperoxidase catalyzes the oxidation of chloride ions to hypochlorus acid, which is a potent antimicrobial agent. Myeloperoxidase pro-oxidative and pro-inflammatory properties cause an increase in MPO in multiple diseases including heart disease, myocardial infraction, and multiple sclerosis. Myeloperoxidase is a clinical marker of myocardial infraction and cardiovascular disease.

    The standard protein in this product is purified from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Human MPO competitor curve comparison
    Human MPO competitor curve comparison

    Standard Curve comparison between Human MPO SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Human MPO standard curve comparison
    Human MPO standard curve comparison

    Standard Curve comparison between the original Human MPO SimpleStep ELISA kit (ab195212) and current Human MPO SimpleStep ELISA kit (ab272101).

  • Example of human MPO standard curve in Sample Diluent NS.
    Example of human MPO standard curve in Sample Diluent NS.

    The MPO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native MPO in human serum, plasma and cell culture supernatant samples.
    Interpolated concentrations of native MPO in human serum, plasma and cell culture supernatant samples.

    The concentrations of MPO were measured in duplicates, interpolated from the MPO standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, plasma (EDTA) 25%, and HL-60 supernatant 1: 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 7.38 ng/mL in serum, 20.34 ng/mL in plasma (citrate), 13.06 ng/ml plasma (EDTA), and 1,035.38 ng/ml in HL-60 supernatant.

  • Interpolated concentrations of native MPO in human HL-60 cell culture extract based on a 12.5 µg/mL extract load.
    Interpolated concentrations of native MPO in human HL-60 cell culture extract based on a 12.5 µg/mL extract load.

    The concentrations of MPO were measured in duplicate and interpolated from the MPO standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 3.69 ng/ml in HL-60 cell culture extract.

  • Serum from ten individual healthy human female donors was measured in duplicate.
    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 20.92 ng/mL with a range of 11.65 – 38.79 ng/mL.

  • Sandwich ELISA - Human Myeloperoxidase ELISA Kit (ab272101)
    Sandwich ELISA - Human Myeloperoxidase ELISA Kit (ab272101)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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