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Developmental Biology Embryogenesis Embryonic stem cells Intracellular

Anti-Nanog antibody [NNG-811] (ab62734)

Price and availability

321 638 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Nanog antibody [NNG-811] (ab62734)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [NNG-811] to Nanog
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Nanog antibody [NNG-811]
    See all Nanog primary antibodies
  • Description

    Mouse monoclonal [NNG-811] to Nanog
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length human Nanog.

  • Positive control

    • Extracts of NT2 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Purified Immunoglobulin
  • Clonality

    Monoclonal
  • Clone number

    NNG-811
  • Isotype

    IgG1
  • Research areas

    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (ab62734)
    Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (ab62734)
    3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 mg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
  • Western blot - Anti-Nanog antibody [NNG-811] (ab62734)
    Western blot - Anti-Nanog antibody [NNG-811] (ab62734)
    Lane 1 : Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/ml
    Lane 2 : Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/ml
    Lane 3 : Negative control – no primary antibody

    All lanes : Cell extract of NT2 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734)
    Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734)

    Nanog was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell extract using ab62734.

    Lane 1: 5 μg ab62734 IP in NT2/D1 whole cell extract.
    Lane 2: 10 μg ab62734 IP in NT2/D1 whole cell extract.
    Lane 3: Negative control without cell extract.
    Lane 4: NT2/D1 whole cell extract (input).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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