Anti-Stra8 antibody (ab49602)
Key features and details
- Rabbit polyclonal to Stra8
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-Stra8 antibody
See all Stra8 primary antibodies -
Description
Rabbit polyclonal to Stra8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Chinese hamster
Predicted to work with: Rat, Hamster
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Immunogen
Synthetic peptide corresponding to Mouse Stra8 aa 1-100.
(Peptide available asab49601) -
Positive control
- IHC-P: Mouse testis tissue. WB: CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab49602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa. Target
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Function
Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. -
Tissue specificity
Expressed specifically in testis. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 20899 Mouse
- Entrez Gene: 500079 Rat
- SwissProt: P70278 Mouse
- Unigene: 5171 Mouse
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Alternative names
- Stimulated by retinoic acid 8 antibody
- stimulated by retinoic acid 8 homolog antibody
- Stimulated by retinoic acid Gene 8 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (ab49602)IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49602, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Stra8 antibody (ab49602)All lanes : Anti-Stra8 antibody (ab49602) at 1 µg/ml
Lane 1 : CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate
Lane 2 : CHO Negative Control Whole Cell Lysate
Lysates/proteins at 1 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa. -
Western blot - Anti-Stra8 antibody (ab49602)This image is courtesy of an anonymous abreview.All lanes : Anti-Stra8 antibody (ab49602) at 1/500 dilution
Lane 1 : WT D3 mouse ES cells cultured with LIF.
Lane 2 : WT D3 mouse ES cells cultured without LIF for 24 hours.
Lane 3 : WT D3 mouse ES cells cultured without LIF and with 1uM Retinoic acid for 24 hours.
Lysates/proteins at 200000 cells per lane.
Secondary
All lanes : Non-Abcam antibody was used: goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Protocols
Datasheets and documents
References (56)
ab49602 has been referenced in 56 publications.
- Omidpanah S et al. Effects of Monoterpenes of Trachyspermum ammi on the Viability of Spermatogonia Stem Cells In Vitro. Plants (Basel) 9:N/A (2020). PubMed: 32182777
- Zheng Y et al. Establishment of cell lines with porcine spermatogonial stem cell properties. J Anim Sci Biotechnol 11:33 (2020). PubMed: 32308978
- Heinrich A et al. Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis. Cell Rep 31:107513 (2020). PubMed: 32294451
- Satirapod C et al. Estrogen regulation of germline stem cell differentiation as a mechanism contributing to female reproductive aging. Aging (Albany NY) 12:7313-7333 (2020). PubMed: 32302290
- Bhattacharyya T et al. Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes. Curr Biol 29:1002-1018.e7 (2019). PubMed: 30853435
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (ab49602)IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49602, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Stra8 antibody (ab49602)All lanes : Anti-Stra8 antibody (ab49602) at 1 µg/ml
Lane 1 : CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate
Lane 2 : CHO Negative Control Whole Cell Lysate
Lysates/proteins at 1 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa. -
Western blot - Anti-Stra8 antibody (ab49602) This image is courtesy of an anonymous abreview.All lanes : Anti-Stra8 antibody (ab49602) at 1/500 dilution
Lane 1 : WT D3 mouse ES cells cultured with LIF.
Lane 2 : WT D3 mouse ES cells cultured without LIF for 24 hours.
Lane 3 : WT D3 mouse ES cells cultured without LIF and with 1uM Retinoic acid for 24 hours.
Lysates/proteins at 200000 cells per lane.
Secondary
All lanes : Non-Abcam antibody was used: goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
