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Developmental Biology Embryogenesis Embryonic stem cells Intracellular

Anti-Nanog antibody [SER211] (ab241542)

Price and availability

307 566 ₸

Availability

Order now and get it on Tuesday March 23, 2021

Anti-Nanog antibody [SER211] (ab241542)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [SER211] to Nanog
  • Suitable for: WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-Nanog antibody [SER211]
    See all Nanog primary antibodies
  • Description

    Rat monoclonal [SER211] to Nanog
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment (GST-tag) within Nanog aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: F9 whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SER211
  • Isotype

    IgG2a
  • Research areas

    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Images

  • Western blot - Anti-Nanog antibody [SER211] (ab241542)
    Western blot - Anti-Nanog antibody [SER211] (ab241542)
    All lanes : Anti-Nanog antibody [SER211] (ab241542) at 1 µg/ml

    Lane 1 : F9 whole cell lysate
    Lane 2 : MEF1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab241542 observed at 40 kDa. Red - loading control, ab52866 (Anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

    ab241542 was shown to react with Nanog in whole cell lysates subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab241542 and ab52866 (Anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/µl and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Nanog antibody [SER211] (ab241542)
    Anti-Nanog antibody [SER211] (ab241542)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-Nanog antibody [EPR20694] - ChIP Grade (ab214549)

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-Nanog antibody [EPR2027(2)] (ab109250)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Alexa Fluor® 647 Anti-Nanog antibody [EPR2027(2)] (ab195018)

    Applications: ICC/IF

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    Anti-Nanog antibody [EPR20694] - BSA and Azide free (ab231300)

    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

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