Anti-Nanog antibody [SER211] (ab241542)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [SER211] to Nanog
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-Nanog antibody [SER211]
See all Nanog primary antibodies -
Description
Rat monoclonal [SER211] to Nanog -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Recombinant fragment (GST-tag) within Nanog aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: F9 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SER211 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-Nanog antibody [SER211] (ab241542) at 1 µg/ml
Lane 1 : F9 whole cell lysate
Lane 2 : MEF1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab241542 observed at 40 kDa. Red - loading control, ab52866 (Anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab241542 was shown to react with Nanog in whole cell lysates subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab241542 and ab52866 (Anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/µl and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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