Anti-Stra8 antibody (ab49405)
Key features and details
- Rabbit polyclonal to Stra8
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Chinese hamster
- Isotype: IgG
Overview
-
Product name
Anti-Stra8 antibody
See all Stra8 primary antibodies -
Description
Rabbit polyclonal to Stra8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Chinese hamster -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse Stra8.
Read Abcam's proprietary immunogen policy (Peptide available as ab49600.) -
Positive control
- WB: Stra8 (mouse) transient overexpression lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab49405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Target
-
Function
Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. -
Tissue specificity
Expressed specifically in testis. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 20899 Mouse
- SwissProt: P70278 Mouse
- Unigene: 5171 Mouse
-
Alternative names
- Stimulated by retinoic acid 8 antibody
- stimulated by retinoic acid 8 homolog antibody
- Stimulated by retinoic acid Gene 8 antibody
see all
Images
-
Western blot - Anti-Stra8 antibody (ab49405)All lanes : Anti-Stra8 antibody (ab49405) at 1 µg/ml
Lane 1 : CHO overexpressing Mouse Stra8 lysate
Lane 2 : CHO Negative Control Lysate
Lysates/proteins at 1 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteAb49405 recognizes tagged overexpressed Stra8 protein which has an expected molecular weight of 60 kDa.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (ab49405)IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49405, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (27)
ab49405 has been referenced in 27 publications.
- Gura MA et al. Dynamic and regulated TAF gene expression during mouse embryonic germ cell development. PLoS Genet 16:e1008515 (2020). PubMed: 31914128
- Vaughan D et al. Inhibition of Activin/Myostatin signalling induces skeletal muscle hypertrophy but impairs mouse testicular development. Eur J Transl Myol 30:8737 (2020). PubMed: 32499882
- Bhattacharyya T et al. Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes. Curr Biol 29:1002-1018.e7 (2019). PubMed: 30853435
- Kojima ML et al. Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice. Elife 8:N/A (2019). PubMed: 30810530
- Todaro F et al. Regulation of Kit Expression in Early Mouse Embryos and ES Cells. Stem Cells 37:332-344 (2019). PubMed: 30566254
Images
-
Western blot - Anti-Stra8 antibody (ab49405)All lanes : Anti-Stra8 antibody (ab49405) at 1 µg/ml
Lane 1 : CHO overexpressing Mouse Stra8 lysate
Lane 2 : CHO Negative Control Lysate
Lysates/proteins at 1 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteAb49405 recognizes tagged overexpressed Stra8 protein which has an expected molecular weight of 60 kDa.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (ab49405)IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49405, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
