Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)
![Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)](https://www.abcam.com/ps/products/251/ab251409/Images/ab251409-406015-anti-nanog-antibody-epr19646-bsa-and-azide-free-western-blot.jpg "Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19646] to Nanog - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Nanog antibody [EPR19646] - BSA and Azide free
See all Nanog primary antibodies -
Description
Rabbit monoclonal [EPR19646] to Nanog - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251409 is the carrier-free version of ab203919. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251409 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19646 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)All lanes : Anti-Nanog antibody [EPR19646] (ab203919) at 1/1000 dilution
Lane 1 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab203919, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.
Negative control: NIH/3T3 (PMID: 12787505).
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![Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)](https://www.abcam.com/ps/products/251/ab251409/Images/ab251409-403390-anti-nanog-antibody-epr19646-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)All lanes : Anti-Nanog antibody [EPR19646] (ab203919) at 1/1000 dilution
Lane 1 : NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 2 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab203919, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.
Negative control: Jurkat (PMID: 12787505).
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![Immunoprecipitation - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)](https://www.abcam.com/ps/products/251/ab251409/Images/ab251409-1-anti-nanog-antibody-epr19646-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)This data was developed using ab203919, the same antibody clone in a different buffer formulation.Nanog was immunoprecipitated from 0.35 mg of F9 (Mouse embryonic testicular cancer cell line) whole cell lysate with ab203919 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203919 at 1/500 dilution. VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: F9 whole cell lysate 10µg (Input). Lane 2: ab203919 IP in F9 whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab203919 in F9 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds. -
![Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)](https://www.abcam.com/ps/products/251/ab251409/Images/ab251409-4-benefits-of-recombinant-antibodies.png)
Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)