Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19646] to Nanog - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Nanog antibody [EPR19646] - BSA and Azide free
    See all Nanog primary antibodies

  • Description

    Rabbit monoclonal [EPR19646] to Nanog - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251409 is the carrier-free version of ab203919. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251409 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)
    Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)
    All lanes : Anti-Nanog antibody [EPR19646] (ab203919) at 1/1000 dilution

    Lane 1 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab203919, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.

    Negative control: NIH/3T3 (PMID: 12787505).

  • Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)
    Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)
    All lanes : Anti-Nanog antibody [EPR19646] (ab203919) at 1/1000 dilution

    Lane 1 : NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 2 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab203919, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.

    Negative control: Jurkat (PMID: 12787505).

  • Immunoprecipitation - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)
    Immunoprecipitation - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)
    This data was developed using ab203919, the same antibody clone in a different buffer formulation.Nanog was immunoprecipitated from 0.35 mg of F9 (Mouse embryonic testicular cancer cell line) whole cell lysate with ab203919 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203919 at 1/500 dilution. VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: F9 whole cell lysate 10µg (Input). Lane 2: ab203919 IP in F9 whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab203919 in F9 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)
    Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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