Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)
![Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)](https://www.abcam.com/ps/products/251/ab251234/Images/ab251234-405766-anti-sts1-antibody-epr16980-bsa-and-azide-free-western-blot.jpg "Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16980] to Sts1 - BSA and Azide free
- Suitable for: WB, ICC, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Sts1 antibody [EPR16980] - BSA and Azide free
See all Sts1 primary antibodies -
Description
Rabbit monoclonal [EPR16980] to Sts1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251234 is the carrier-free version of ab197027. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251234 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16980 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)All lanes : Anti-Sts1 antibody [EPR16980] (ab197027) at 1/2000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaThis data was developed using ab197027, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)](https://www.abcam.com/ps/products/251/ab251234/Images/ab251234-2-anti-sts1-antibody-epr16980-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)This data was developed using ab197027, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Sts1 with purified ab197027 at 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. -
![Immunocytochemistry - Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)](https://www.abcam.com/ps/products/251/ab251234/Images/ab251234-1-anti-sts1-antibody-epr16980-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)This data was developed using ab197027, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Sts1 with ab197027 at 1/280 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and weakly nuclear staining on Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab197027 at 1/280 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)](https://www.abcam.com/ps/products/251/ab251234/Images/ab251234-4-benefits-of-recombinant-antibodies.png)
Anti-Sts1 antibody [EPR16980] - BSA and Azide free (ab251234)