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Anti-Nanog antibody [23D2-3C6] (ab173368)

Key features and details

  • Mouse monoclonal [23D2-3C6] to Nanog
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Nanog antibody [23D2-3C6]
    See all Nanog primary antibodies

  • Description

    Mouse monoclonal [23D2-3C6] to Nanog

  • Host species

    Mouse

  • Specificity

    ab173368 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. It shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Nanog aa 1-305. expressed in bacteria.
    Sequence:

    MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV


    Database link: Q9H9S0
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • NTERA-2 cells; HEL 11.4 induced IPS cells; H9 embryonic stem cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)
    Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Immunofluorescent analysis of H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green)  followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.

  • Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)
    Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)
    All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

    Lane 1 : NCCIT nuclear fraction lysate
    Lane 2 : NCCIT cytoplasmic fraction lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : goat anti-mouse IgG-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    4-20% Tris-HCl polyacrylamide gel.

  • Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)
    Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)
    All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

    Lane 1 : NCCIT lysate
    Lane 2 : NTERA-2 lysate
    Lane 3 : HeLa lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : goat anti-mouse IgG-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    4-20% Tris-HCl polyacrylamide gel

  • Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)
    Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Flow cytometric analysis of H9 embryonic stem cells labeling Nanog with ab173368 at 1/100 (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary at 1/200 dilution. 

  • Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)
    Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Flow cytometric analysis of HEL 11.4 induced IPS cells labeling Nanog with ab173368 at 1/00 dilution (blue histogram) or mouse IgG (green histogram)  followed by a fluorescein-conjugated secondary antibody at 1/200 dilution. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)
    Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Immunofluorescent analysis of HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green)  followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)
    Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Immunofluorescent analysis of NTERA-2 and HeLa cells (formalin-fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/50 dilution (green) followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

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