Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nanog antibody [SER211] - BSA and Azide free (ab252789)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [SER211] to Nanog - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Nanog antibody [SER211] - BSA and Azide free
    See all Nanog primary antibodies

  • Description

    Rat monoclonal [SER211] to Nanog - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment (GST-tag) within Nanog aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: F9 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252789 is the carrier-free version of ab241542. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Nanog antibody [SER211] - BSA and Azide free (ab252789)
    Western blot - Anti-Nanog antibody [SER211] - BSA and Azide free (ab252789)
    All lanes : Anti-Nanog antibody [SER211] (ab241542) at 1 µg/ml

    Lane 1 : F9 whole cell lysate
    Lane 2 : MEF1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 34 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab241542 observed at 40 kDa. Red - loading control, ab52866 (Anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

    ab241542 was shown to react with Nanog in whole cell lysates subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab241542 and ab52866 (Anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/µl and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    This image was produced using the same clone but in a different buffer, ab241542; PBS, 40% Glycerol, 0.05% BSA and 0.02% sodium azide.

  • Anti-Nanog antibody [SER211] - BSA and Azide free (ab252789)
    Anti-Nanog antibody [SER211] - BSA and Azide free (ab252789)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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