Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23994-103] to Calcium-independent Phospholipase A2/PLA2G6
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103]
    See all Calcium-independent Phospholipase A2/PLA2G6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23994-103] to Calcium-independent Phospholipase A2/PLA2G6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse testis tissue; Rat testis tissue lysates; F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate; C6 (rat glial tumor glial cell), whole cell lysate. IHC-P: Mouse testis; Rat testis.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23994-103

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
    All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate at 20 µg
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse lung tissue lysate 20ug

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
    Lanes 2-3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa
    Observed band size: 36, 88 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 29109765)

    Low expression: brain, lung (PMID:10336645)

    Exposure time: 10 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Calcium-independent Phospholipase A2/PLA2G6 with ab259950 at 1/100 (8.18 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse testis (PMID: 9079687). The section was incubated with ab259950 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
    All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1 : Rat testis tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa
    Observed band size: 36, 88 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID:29109765)

    Low expression: brain, lung (PMID:10336645)

    Exposure time: 10 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Calcium-independent Phospholipase A2/PLA2G6 with ab259950 at 1/100 (8.18 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat testis (PMID: 9079687). The section was incubated with ab259950 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
    All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
    Lane 2 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa
    Observed band size: 88 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The blot was developed using a higher sensitivity ECL substrate.

     Exposure time: 3 minutes

     

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