Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)
![Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)](https://www.abcam.com/ps/products/259/ab259950/Images/ab259950-450357-anti-calciumindependent-phospholipase-a2pla2g6-antibody-epr23994103-western-blot-lysate.jpg "Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23994-103] to Calcium-independent Phospholipase A2/PLA2G6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103]
See all Calcium-independent Phospholipase A2/PLA2G6 primary antibodies -
Description
Rabbit monoclonal [EPR23994-103] to Calcium-independent Phospholipase A2/PLA2G6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse testis tissue; Rat testis tissue lysates; F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate; C6 (rat glial tumor glial cell), whole cell lysate. IHC-P: Mouse testis; Rat testis.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23994-103 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate at 20 µg
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse lung tissue lysate 20ug
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Lanes 2-3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 89 kDa
Observed band size: 36, 88 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 29109765)
Low expression: brain, lung (PMID:10336645)
Exposure time: 10 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)](https://www.abcam.com/ps/products/259/ab259950/Images/ab259950-1-anti-calciumindependent-phospholipase-a2pla2g6-antibody-epr23994103-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Calcium-independent Phospholipase A2/PLA2G6 with ab259950 at 1/100 (8.18 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse testis (PMID: 9079687). The section was incubated with ab259950 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)](https://www.abcam.com/ps/products/259/ab259950/Images/ab259950-450359-anti-calciumindependent-phospholipase-a2pla2g6-antibody-epr23994103-western-blot-lysate1.jpg)
Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 89 kDa
Observed band size: 36, 88 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID:29109765)
Low expression: brain, lung (PMID:10336645)
Exposure time: 10 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)](https://www.abcam.com/ps/products/259/ab259950/Images/ab259950-2-anti-calciumindependent-phospholipase-a2pla2g6-antibody-epr23994103-immunohistochemistry-rat-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Calcium-independent Phospholipase A2/PLA2G6 with ab259950 at 1/100 (8.18 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat testis (PMID: 9079687). The section was incubated with ab259950 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)](https://www.abcam.com/ps/products/259/ab259950/Images/ab259950-450360-anti-calciumindependent-phospholipase-a2pla2g6-antibody-epr23994103-western-blot-carcinoma.jpg)
Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950)All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution
Lane 1 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
Lane 2 : C6 (rat glial tumor glial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 89 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
