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Signal Transduction Protein Phosphorylation Tyrosine Kinases Src Family

Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)

Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6771] to CSK - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-CSK antibody [EPR6771] - BSA and Azide free
    See all CSK primary antibodies
  • Description

    Rabbit monoclonal [EPR6771] to CSK - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248095 is the carrier-free version of ab125005.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 5.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6771
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Neuroscience
    • Development

Images

  • Western blot - Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
    Western blot - Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)

    This data was developed using ab125005, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: CSK knockout HAP1 cell lysate (20 µg)

    Lane 3: Ramos cell lysate (20 µg)

    Lane 4: Jurkat cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab125005 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab125005 was shown to specifically react with CSK when CSK knockout samples were used. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab125005 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
    Western blot - Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
    All lanes : Anti-CSK antibody [EPR6771] (ab125005) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 50 kDa



    This data was developed using ab125005, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
    This data was developed using ab125005, the same antibody clone in a different buffer formulation.ab125005 at 1/50 dilution staining CSK in paraffin-embedded Human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
    OI-RD Scanning - Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
    This data was developed using ab125005, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)
    Anti-CSK antibody [EPR6771] - BSA and Azide free (ab248095)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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