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Signal Transduction Protein Phosphorylation Tyrosine Kinases Src Family

Anti-Lyn antibody [Y497] - BSA and Azide free (ab239813)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Lyn antibody [Y497] - BSA and Azide free (ab239813)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y497] to Lyn - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Lyn antibody [Y497] - BSA and Azide free
    See all Lyn primary antibodies
  • Description

    Rabbit monoclonal [Y497] to Lyn - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    Residues close to SH3 domain of human Lyn
  • Positive control

    • WB: HAP1, Ramos, PC-12, and A431 whole cell lysates. Mouse lung, mouse and rat liver tissue lysates.
  • General notes

    Ab239813 is the carrier-free version of ab32398. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239813 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y497
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family

Images

  • Western blot - Anti-Lyn antibody [Y497] - BSA and Azide free (ab239813)
    Western blot - Anti-Lyn antibody [Y497] - BSA and Azide free (ab239813)
    All lanes : Anti-Lyn antibody [Y497] (ab32398) at 1/1000 dilution (Purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32398, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Lyn antibody [Y497] - BSA and Azide free (ab239813)

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    Anti-Lyn antibody (ab137338)

    Applications: ICC/IF, IHC-P, WB

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    Anti-Lyn antibody [Y497] (ab32398)

    Applications: WB

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    Anti-Lyn (phospho Y396) antibody (ab226778)

    Applications: WB

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    Applications: Flow Cyt, WB

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    Anti-Lyn antibody (ab226802)

    Applications: WB

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    Anti-Lyn (phospho Y507) antibody [EP504Y] - BSA and Azide free (ab256224)

    Applications: Dot, IP, WB

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    Anti-Lyn antibody (ab227170)

    Applications: ICC/IF, IP, WB

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    Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)

    Applications: Dot, IP, WB

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