Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free (ab209615)
![Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free (ab209615)](https://www.abcam.com/ps/products/209/ab209615/Images/ab209615-2-ab185617237206FC.jpg "Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free (ab209615)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17734] to Src (phospho Y419) - BSA and Azide free
- Suitable for: Dot blot, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free
See all Src primary antibodies -
Description
Rabbit monoclonal [EPR17734] to Src (phospho Y419) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, NIH/3T3 and C6 whole cell lysates treated with 50mM pervanadate for 5 minutes. Flow Cytometry: NIH/3T3 cells treated with 50mM pervanadate for 30 minutes.
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General notes
Ab209615 is the carrier-free version of ab185617. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab209615 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17734 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free (ab209615)
Flow cytometric analysis of 2% paraformaldehyde-fixed NIH/3T3 (Mouse embyro fibroblast cells) cells, treated with 50mM Pervanadate for 30 minutes, labeling Src (phospho Y419) with ab185617 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. NIH/3T3 cells treated with 50mM Pervanadate for 30 min were also preincubated with non phospho peptide (orange) and with phospho peptide (purple).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185617).
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![Dot Blot - Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free (ab209615)](https://www.abcam.com/ps/products/209/ab209615/Images/ab209615-1-ab185617237205dottblot.jpg)
Dot Blot - Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free (ab209615)Dot blot analysis of Src (phospho Y419) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab185617 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185617).
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![Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free (ab209615)](https://www.abcam.com/ps/products/209/ab209615/Images/ab209615-3-benefits-of-recombinant-antibodies.png)
Anti-Src (phospho Y419) antibody [EPR17734] - BSA and Azide free (ab209615)
