Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Src (phospho Y419) antibody [EPR17734] (ab185617)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17734] to Src (phospho Y419)
Suitable for: WB, Flow Cyt, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-Src (phospho Y419) antibody [EPR17734]
See all Src primary antibodies
Description

Rabbit monoclonal [EPR17734] to Src (phospho Y419)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A431, NIH/3T3 and C6 whole cell lysates treated with 50mM pervanadate for 5 minutes. Flow Cytometry: NIH/3T3 cells treated with 50mM pervanadate for 30 minutes.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17734
Isotype

IgG
Research areas

Western blot - Anti-Src (phospho Y419) antibody [EPR17734] (ab185617)

All lanes : Anti-Src (phospho Y419) antibody [EPR17734] (ab185617) at 1/5000 dilution

Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysate treated with 50mM pervanadate for 5 minutes
Lane 2 : Untreated A431 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Src (phospho Y419) antibody [EPR17734] (ab185617)

All lanes : Anti-Src (phospho Y419) antibody [EPR17734] (ab185617) at 1/5000 dilution

Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate treated with 50mM pervanadate for 5 minutes
Lane 2 : Untreated NIH/3T3 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Src (phospho Y419) antibody [EPR17734] (ab185617)

All lanes : Anti-Src (phospho Y419) antibody [EPR17734] (ab185617) at 1/5000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysate treated with 50mM pervanadate for 5 minutes
Lane 2 : Untreated C6 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-Src (phospho Y419) antibody [EPR17734] (ab185617)

Flow cytometric analysis of 2% paraformaldehyde-fixed NIH/3T3 (Mouse embyro fibroblast cells) cells, treated with 50mM Pervanadate for 30 minutes, labeling Src (phospho Y419) with ab185617 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. NIH/3T3 cells treated with 50mM Pervanadate for 30 min were also preincubated with non phospho peptide (orange) and with phospho peptide (purple).
Dot Blot - Anti-Src (phospho Y419) antibody [EPR17734] (ab185617)

Dot blot analysis of Src (phospho Y419) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab185617 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Src (phospho Y419) antibody [EPR17734] (ab185617)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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