Anti-YAP1 antibody (ab226817)
Key features and details
- Rabbit polyclonal to YAP1
- Suitable for: WB, IP
- Reacts with: Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-YAP1 antibody
See all YAP1 primary antibodies -
Description
Rabbit polyclonal to YAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB HumanZebrafish
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Immunogen
Synthetic peptide within Human YAP1 (N terminal). The exact sequence is proprietary.
Database link: P46937 -
Positive control
- WB: A431, HeLa and HepG2 cell lysate; zebrafish brain and eye tissue lysate. IP: HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-YAP1 antibody (ab226817)All lanes : Anti-YAP1 antibody (ab226817) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa transfected with YAP1 shRNA cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
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Western blot - Anti-YAP1 antibody (ab226817)All lanes : Anti-YAP1 antibody (ab226817) at 1/1000 dilution
Lane 1 : Zebrafish brain tissue lysate
Lane 2 : Zebrafish eye tissue lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
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Immunoprecipitation - Anti-YAP1 antibody (ab226817)YAP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab226817. Western blot was performed from the immunoprecipitate using ab226817 at 1/100 dilution.
Lane 1: Rabbit IgG instead of ab226817 in HeLa whole cell lysate.
Lane 2: ab226817 IP in HeLa whole cell lysate. -
Western blot - Anti-YAP1 antibody (ab226817)All lanes : Anti-YAP1 antibody (ab226817) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
