Anti-YAP1 (phospho Y357) antibody (ab62751)
Key features and details
- Rabbit polyclonal to YAP1 (phospho Y357)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-YAP1 (phospho Y357) antibody
See all YAP1 primary antibodies -
Description
Rabbit polyclonal to YAP1 (phospho Y357) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Opossum -
Immunogen
Synthetic phosphopeptide: GLSMSSYPSVPRT conjugated to KLH, corresponding to C-terminal amino acids 351-362 (phosho Y357) of Human YAP1.
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Positive control
- HEK-293T cells co-transfected with human YAP1 and human c-Abl.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab62751 was affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-YAP1 (phospho Y357) antibody (ab62751) at 1/2000 dilution
Lane 1 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl
Lane 2 : Whole cell lysates of untransfected HEK-293T cells
Lane 3 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with YAP1 peptide (human 351-362) at 20 µg/ml
Lane 4 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with phospho-YAP1 (human 351-362 [phospho Y357]) immunizing peptide at 20 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.