Anti-Src (phospho S75) antibody (ab79308)
Key features and details
- Rabbit polyclonal to Src (phospho S75)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Src (phospho S75) antibody
See all Src primary antibodies -
Description
Rabbit polyclonal to Src (phospho S75) -
Host species
Rabbit -
Specificity
Detects Src only when phosphorylated at serine 75 -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic phosphopeptide derived from Human Src around the phosphorylation site of serine 75 (VTSPPQ)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Src (phospho S75) antibody (ab79308)All lanes : Anti-Src (phospho S75) antibody (ab79308) at 1/1000 dilution
Lane 1 : extracts from COLO cells (treated
with EGF, 200ng/ml, 30mins)
Lane 2 : extracts from COLO cells (treated
with EGF, 200ng/ml, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho S75) antibody (ab79308)Left panel: immunohistochemistry analysis of paraffin-embedded Human brain tissue with ab79308 at 1/100 dilution. Right panel: same, after pre-incubation with the immunizing peptide -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho S75) antibody (ab79308) This image is courtesy of an anonymous Abreviewab79308 staining Src (phospho S75) in Dog osteosarcoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with acetone and blocked with 2% serum for 1 hour; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50 in serum) for 14 hours at 4°C. A Biotinylated Goat anti-rabbit IgG (1/400) was used as the secondary antibody.
