Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSK antibody [EPR6771] (ab125005)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6771] to CSK
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CSK antibody [EPR6771]
    See all CSK primary antibodies

  • Description

    Rabbit monoclonal [EPR6771] to CSK

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CSK aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Jurkat, Ramos and RAW 264.7 cell lysates; Human tonsil tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 5.10 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6771

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CSK antibody [EPR6771] (ab125005)
    Western blot - Anti-CSK antibody [EPR6771] (ab125005)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CSK knockout HAP1 cell lysate (20 µg)
    Lane 3: Ramos cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab125005 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab125005 was shown to specifically react with CSK when CSK knockout samples were used. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab125005 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-CSK antibody [EPR6771] (ab125005)
    Western blot - Anti-CSK antibody [EPR6771] (ab125005)
    All lanes : Anti-CSK antibody [EPR6771] (ab125005) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody [EPR6771] (ab125005)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody [EPR6771] (ab125005)

    ab125005 at 1/50 dilution staining CSK in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CSK antibody [EPR6771] (ab125005)
    OI-RD Scanning - Anti-CSK antibody [EPR6771] (ab125005)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CSK antibody [EPR6771] (ab125005)
    Anti-CSK antibody [EPR6771] (ab125005)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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