Anti-YAP1 antibody (ab245286)
Key features and details
- Rabbit polyclonal to YAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-YAP1 antibody
See all YAP1 primary antibodies -
Description
Rabbit polyclonal to YAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human YAP1 aa 250-300. The exact sequence is proprietary. NP_006097.1
Database link: P46937 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245286 was affinity purified using an epitope specific to YAP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-YAP1 antibody (ab245286)All lanes : Anti-YAP1 antibody (ab245286) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 54 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-YAP1 antibody (ab245286)YAP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245286 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245286 at 1 µg/ml.
Lane 1: ab245286 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
