Anti-Src (phospho Y418) antibody (ab47411)
Key features and details
- Rabbit polyclonal to Src (phospho Y418)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Src (phospho Y418) antibody
See all Src primary antibodies -
Description
Rabbit polyclonal to Src (phospho Y418) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Src only when phosphorylated at tyrosine 418. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human Src around the phosphorylation site of tyrosine 418 (N-E-YP-T-A).
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Positive control
- Human colon carcinoma tissue; COLO205 cell extracts
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y418) antibody (ab47411)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue in the presence (right) and absence (left) of blocking phosphopeptide, using ab47411 at 1/50 dilution.
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Western blot - Anti-Src (phospho Y418) antibody (ab47411)All lanes : Anti-Src (phospho Y418) antibody (ab47411) at 1/500 dilution
Lane 1 : COLO205 cell extracts with serum
Lane 2 : COLO205 cell extracts
Predicted band size: 60 kDa
Western blot analysis of extracts from COLO205 cells in the presence (lane 2) and absence (lane 1) of serum, using ab47411 at 1/500 dilution.
