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Mouse CCL27 ELISA Kit (CTACK) (ab202406)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 9.3 pg/ml
  • Range: 23.4 pg/ml - 1500 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse CCL27 ELISA Kit (CTACK)
    See all CCL27 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall 8 5.34%
    Inter-assay
    Sample n Mean SD CV%
    overall 3 10.94%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    9.3 pg/ml

  • Range

    23.4 pg/ml - 1500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 110 100% - 116%
    Cell culture media 116 103% - 128%
    Hep Plasma 97 71% - 114%
    EDTA Plasma 128 105% - 146%
    Cit plasma 107 81% - 124%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse CCL27 ELISA Kit (CTACK) (ab202406) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL27 (CTACK) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL27 (CTACK) with 9.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    CCL27 is a chemotactic factor that attracts skin-associated memory T-lymphocytes, and binds to CCR10. It may play a role in cell migration during embryogenesis, and may mediate lymphocyte homing to cutaneous sites. Nuclear forms of CCL27 may facilitate cellular migration by inducing cytoskeletal relaxation. There is evidence that CCL27 is strongly upregulated in atopic dermatitis, contact dermatitis, and psoriasis skin lesions.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse CCL27 ELISA Kit (CTACK) (ab202406)
    Other - Mouse CCL27 ELISA Kit (CTACK) (ab202406)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of CCL27 standard curve.
    Example of CCL27 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.
    Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.

    The 1X dilution was 3.13% for mouse serum, 6.25% for citrate and EDTA plasma, and 1.56% for heparin plasma. Interpolated data values (mean +/- SD, n = 2) are graphed.

  • CCL27 levels in stimulated (1.5% PHA + 10 ng/mL PMA, 72 hrs (+)) and unstimulated (-) mouse L929 supernatants.
    CCL27 levels in stimulated (1.5% PHA + 10 ng/mL PMA, 72 hrs (+)) and unstimulated (-) mouse L929 supernatants.

    A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 100% for both the stimulated and unstimulated supernatants. Interpolated data values (mean +/- SD, n = 2) are graphed.

  • Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (ab202406)
    Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (ab202406)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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