Mouse CCL27 Matched Antibody Pair Kit (ab212064)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 31.3 pg/ml - 2000 pg/ml
Overview
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Product name
Mouse CCL27 Matched Antibody Pair Kit
See all CCL27 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
5.117 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CCL27 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CCL27.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab202406. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse CCL27 Capture Antibody 2 x 50µg 1 x 50µg Mouse CCL27 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse CCL27 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10. -
Tissue specificity
Testis, thymus, placenta, ovary and skin. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ALP
- C-C motif chemokine 27
- CC chemokine ILC
see all -
Database links
- Entrez Gene: 100039863 Mouse
- Entrez Gene: 100039939 Mouse
- Entrez Gene: 100040048 Mouse
- Entrez Gene: 20301 Mouse
- SwissProt: Q9Z1X0 Mouse
- Unigene: 335946 Mouse
- Unigene: 425176 Mouse
- Unigene: 484274 Mouse
see all
Images
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Mouse CCL27 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse CCL27 Matched Antibody Pair Kit (ab212064)To learn more about the advantages of recombinant antibodies see here.
