Human CXCL10 (IP10) knockout A549 cell lysate (ab256888)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263518 - Human CXCL10 knockout A549 cell lysate - Brefeldin A treated 1 x 100µg ab277648 - Human CXCL10 knockout A549 cell lysate - IFN-y, TNF-alpha + Brefeldin A treated 1 x 100µg ab277649 - Human wild-type A549 cell lysate - Brefeldin A treated 1 x 100µg ab277650 - Human wild-type A549 cell lysate - IFN-y, TNF-alpha + Brefeldin A treated 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human CXCL10 knockout HeLa cell lysate
Lane 1: Wild-type A549 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate (30 ug)
Lane 2: Wild-type A549 IFN-y (ab259377) (100 ng/ml, 32 h) and TNF-alpha (ab259410) (10 ng/ml, 32h), and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate (30 ug)
Lane 3: IP10 knockout A549 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate (30 ug)
Lane 4: IP10 knockout A549 IFN-y (ab259377) (100ng/ml, 32h) and TNF-alpha (ab259410) (10ng/ml, 32h), and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate (30 ug)
Lane 5: THP-1 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate (30 ug)
Lane 6: THP-1 IFN-y (ab259377) (200ng/ml, 24h) and LPS (50ng/ml, 24h)-treated for 24 hours, and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate (30 ug)Lanes 1 - 6: Merged signal (red and green). Green - ab214668 observed at 11 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab214668 was shown to react with IP10 in wild-type A549 cells in western blot with loss of signal observed in IP10 knockout cell line ab266971 (knockout cell lysate ab256888). Wild-type and IP10 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab214668 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human CXCL10 knockout A549 cell lysate (ab256888)Allele-1: 4 bp deletion in exon2
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Sanger Sequencing - Human CXCL10 knockout A549 cell lysate (ab256888)Allele-2: 1 bp insertion in exon2
