Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human FAK ELISA Kit (ab187395)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 398 pg/ml
  • Range: 1.56 ng/ml - 100 ng/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human FAK ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 5 5.9%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 3 9.7%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    398 pg/ml

  • Range

    1.56 ng/ml - 100 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 100 99% - 100%
    Extraction Buffer 101 100% - 103%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human FAK ELISA Kit (ab187395) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FAK protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FAK with 398 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Focal Adhesion Kinase (FAK) is a non-receptor protein-tyrosine kinase that is implicated in signaling pathways involved in cell motility, proliferation and apoptosis. FAK is widely expressed in adult tissues as well as being critical during development (angiogenesis, heart and nervous system development). Post-translational modifications include phosphorylation at residue Tyrosine 397. Phosphorylation at this residue promotes interaction with SRC family members and signal transduction (including activation of AKT, MAPK and Rho GTPase cascades); dephosphorylation at this residue promotes focal adhesion disassembly. FAK overexpression and increased kinase activity is observed in many types of cancer.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

Images

  • Other - Human FAK ELISA Kit (ab187395)
    Other - Human FAK ELISA Kit (ab187395)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of FAK standard curve.
    Example of FAK standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of HEPG2 and MCF7 extracts within the working range of the assay.
    Titration of HEPG2 and MCF7 extracts within the working range of the assay.

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Quantitation of FAK in different cell lines
    Quantitation of FAK in different cell lines

    Interpolated values of FAK are plotted for the indicated cell lines based on an extract load of 125 µg/mL.

  • Interpolated concentrations of FAK in HeLa Extract.
    Interpolated concentrations of FAK in HeLa Extract.

    The concentrations of FAK protein were interpolated from the FAK standard curve and corrected for sample dilution relative to extract load of 62.5 µg/mL. The mean FAK concentration was determined to be 44 ng/mL in HeLa extract at 62.5 µg/mL.

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