Anti-EEA1 antibody (ab50313)
Key features and details
- Rabbit polyclonal to EEA1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EEA1 antibody
See all EEA1 primary antibodies -
Description
Rabbit polyclonal to EEA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human -
Immunogen
Synthetic peptide:
GSQGSDLDSSATPINTVDVNNESSSEG
conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 24 - 40 of Human EEA1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313."
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ab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.