Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4245] to EEA1 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EEA1 antibody [EPR4245] - BSA and Azide free
    See all EEA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4245] to EEA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: COS-1, NIH 3T3, C6, HeLa, Jurkat, Daudi, SH-SY5Y and JAR cell lysates. ICC/IF: JAR cells.

  • General notes

    Ab239942 is the carrier-free version of ab109110. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)
    Western blot - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)
    All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : EEA1 knockout HeLa cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 162 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab109110).

    Lanes 1- 4: Merged signal (red and green). Green - ab109110 observed at 175 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab109110 was shown to react with EEA1 in wild-type HeLa cells in western blot. The band observed in knockout cell line ab261822 (knockout cell lysate ab256897) lane below 175kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EEA1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109110 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)
    Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)

    Immunocytochemistry/Immunofluorescence analysis of JAR (human placenta choriocarcinoma epithelial) cells labelling EEA1 with ab109110 at a dilution of 1/250. Cells were fixed with 4% paraformaldehye and permeabilized with 0.1% TritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody at a dilution of 1/1000. Counterstained with DAPI and ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), at a dilution of 1/200.

    Image shows cytoplasmic staining in JAR cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109110).

  • Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)
    Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-EEA1 antibody [EPR4245] - BSA and Azide free (ab239942)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.