Anti-EEA1 antibody (ab264245)
Key features and details
- Rabbit polyclonal to EEA1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-EEA1 antibody
See all EEA1 primary antibodies -
Description
Rabbit polyclonal to EEA1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human EEA1 aa 100-150. The exact sequence is proprietary. NP_003557.2
Database link: Q15075 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-EEA1 antibody (ab264245)All lanes : Anti-EEA1 antibody (ab264245) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
Exposure time: 30 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEA1 antibody (ab264245)Formalin-fixed, paraffin-embeded human breast carcinoma tissue stained for EEA1 using ab264245 at 1/200 dilution in immunohistochemical analysis. DAB staining.
-
Immunoprecipitation - Anti-EEA1 antibody (ab264245)EEA1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264245 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264245 at 1 μg/ml.
Lane 1: ab264245 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 3 seconds.
