Human LAMP1 ELISA Kit (ab277464)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 1.229 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human LAMP1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 ng/ml -
Range
1.229 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89.24 69% - 114% Plasma 92.01 76% - 102% Cell culture media 116.9 100% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human LAMP1 ELISA Kit (ab277464) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LAMP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human LAMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human LAMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human LAMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human LAMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 350X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human LAMP1 Antibody 2 vials Anti-Human LAMP1 coated Microplate 1 unit Human LAMP1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Alternative names
- CD107 antigen like family member A
- CD107 antigen-like family member A
- CD107a
see all -
Database links
- Entrez Gene: 3916 Human
- Omim: 153330 Human
- SwissProt: P11279 Human
- Unigene: 494419 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.