Anti-ISL-2 antibody (ab156621)
Key features and details
- Rabbit polyclonal to ISL-2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ISL-2 antibody
See all ISL-2 primary antibodies -
Description
Rabbit polyclonal to ISL-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Mouse ISL-2 aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: Q9CXV0 -
Positive control
- This antibody gave a positive signal in the following Mouse tissue lysates: E10 Embryonic Brain; E11 Embryonic Brain; E12 Embryonic Brain; E16 Embryonic Brain.
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General notes
Previously labelled as Islet 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications RatCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).Target
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Function
Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 786913 Cow
- Entrez Gene: 64843 Human
- Entrez Gene: 104360 Mouse
- Entrez Gene: 57233 Rat
- Omim: 609481 Human
- SwissProt: Q96A47 Human
- SwissProt: Q9CXV0 Mouse
- SwissProt: P50480 Rat
see all -
Alternative names
- FLJ10160 antibody
- Insulin gene enhancer protein ISL 2 antibody
- Insulin gene enhancer protein ISL-2 antibody
see all
Images
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Western blot - Anti-ISL-2 antibody (ab156621)All lanes : Anti-ISL-2 antibody (ab156621) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : E11 Mouse Embryo Brain Tissue Lysate
Lane 3 : E12 Ms Embryo Brain Tissue Lysate
Lane 4 : E16 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 150 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156621 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab156621 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ISL-2 antibody (ab156621)All lanes : Anti-ISL-2 antibody (ab156621) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : E11 Mouse Embryo Brain Tissue Lysate
Lane 3 : E12 Ms Embryo Brain Tissue Lysate
Lane 4 : E16 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 150 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156621 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
