Anti-c-Kit antibody (ab114992)
Key features and details
- Rabbit polyclonal to c-Kit
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Kit antibody
See all c-Kit primary antibodies -
Description
Rabbit polyclonal to c-Kit -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region between amino acids 925-976 of Human c-Kit (NP_000213.1).
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Positive control
- Purchase matching WB positive control:Active human c-Kit protein fragment
- Human erythroleukemia (HEL) and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
affinity purified using an epitope specific to c-Kit immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody (ab114992)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling c-Kit with ab114992 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-c-Kit antibody (ab114992)All lanes : Anti-c-Kit antibody (ab114992) at 0.1 µg/ml
Lane 1 : HEL whole cell lysate at 50 µg
Lane 2 : HEL whole cell lysate at 15 µg
Lane 3 : HEL whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-c-Kit antibody (ab114992)Lane 1: ab114992 at 1 µg/ml staining c-Kit in HEL whole cell lysate immunoprecipitated using ab114992 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 109 kDa. Exposure time: 3 seconds
